Support Materials

•  Goal - Chapters 5 and 6:
  To know and characterize structure / function relationships of enzymes.
   
•  Objectives:
  Students will be able to...
  • explain how a regulatory enzyme is regulated - allosterism, covalent modification.
  • describe the mechanism of chymotrypsin, a serine protease. In particular, understand the use of arrows to make and break bonds. How is proton transfer used to make a better nucleophile? How is proton transfer used to make a better leaving group? - Fig 6.13.
    Serine proteases catalyze the hydrolysis of peptide bonds (amides). It's another example of a nucleophilic substitution (see phospholipase).
  • explain the role of Zn(II) in carboxypeptidase? - p. 179. Is it the same as the role of Zn(II) in carbonic anhydrase?
  • summarize the function and regulation of glycogen phosphorylase, a regulatory enzyme found in the muscle and the liver. This enzyme releases glucose from stored glycogen during times of energy need - Fig 6.9.